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Image Search Results
Journal: bioRxiv
Article Title: A Rapid Bead-Based Assay For Screening Of SARS-CoV-2 Neutralising Antibodies
doi: 10.1101/2021.10.13.464050
Figure Lengend Snippet: a: GFP nanobody with GST tag immobilized on Sepharose beads were incubated with HEK 293T ACE2-GFP lysate b: HEK 293T cells stably expressing SARS-CoV-2 S1-OFP c: whole-cell extract from HEK293T S1-OFP stable cells readily bind to the ACE2-GFP Beads d: whole-cell extract from HEK293T-S1-OFP cells at a concentration of 100 ug/ml incubated with RFP Trap beads for indicated time points, Respective overlay and RFP channels are shown e: Quantification of the S1-OFP fluorescence intensity at indicated time points are shown f: The S1-OFP beads incubated with whole cell lysate from HEK293T-ACE2-GFP cells at a concentration of 100ug/ml for indicated time points, respective overlay, GFP channel and RFP channels are shown g: Quantification of the ACE2-GFP fluorescence intensity at indicated time points are shown h: The S1-OFP beads were pre-incubated with the indicated dilution of neutralizing antibody for 1 hour followed by ACE2 GFP extract, respective overlay, GFP channel, and RFP channels are shown i: Quantification of the ACE2-GFP fluorescence intensity at indicated dilutions of neutralizing antibody are shown. p-value calculated using Two-way ANOVA with Tukey’s Post hoc hypothesis (0.0001>***,0.001>**, 0.01>*)
Article Snippet: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with
Techniques: Incubation, Stable Transfection, Expressing, Concentration Assay, Fluorescence
Journal: bioRxiv
Article Title: A Rapid Bead-Based Assay For Screening Of SARS-CoV-2 Neutralising Antibodies
doi: 10.1101/2021.10.13.464050
Figure Lengend Snippet: a: HEK293 cells stably expressing a soluble version of ACE2-GFP : HEK293 cells stably expressing a soluble version of S-OFP : Supernatant of soluble ACE2-GFP and S1-OFP stable cells act as ready to use reagents for HTS analysis and the quantification of the ACE2-GFP fluorescence intensity also shown : Quantification of the ACE2-GFP fluorescence intensity at indicated dilution of neutralizing antibody is shown. p-value calculated using student’s t-test (0.0001>***,0.001>**, 0.01>*) : Westernblotting of supernatant from HEK293T cells stably expressing soluble versions of ACE2-GFP, S1-OFP and RBD-His
Article Snippet: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with
Techniques: Stable Transfection, Expressing, Fluorescence
Journal: bioRxiv
Article Title: A Rapid Bead-Based Assay For Screening Of SARS-CoV-2 Neutralising Antibodies
doi: 10.1101/2021.10.13.464050
Figure Lengend Snippet: a: Real-time confocal imaging of HEK293T HA-ACE2 cells and HEK293T WT cells treated with S1-OFP and selected time points are shown b: Life-time imaging of ACE2-GFP bound to S1-OFP beads c: Quantification of life-time images of ACE-GFP beads and ACE2-GFP bound to S1-OFP beads. p-value calculated using student’s t-test (0.0001>***,0.001>**, 0.01>*)
Article Snippet: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with
Techniques: Imaging
Journal: bioRxiv
Article Title: A Rapid Bead-Based Assay For Screening Of SARS-CoV-2 Neutralising Antibodies
doi: 10.1101/2021.10.13.464050
Figure Lengend Snippet: a: Validation of the S1-OFP-ACE2-GFP bead assay using diverse antibodies against SARS-CoV-2-S1 b: pseudovirion assay using diverse antibodies against SARS-CoV-2-S1 c: Quantification of the ACE2-GFP fluorescence intensity of S1-OFP beads incubated with indicated dilutions of antibodies d: Quantification of the pseudovirion infection (ZsGreen fluorescence intensity) incubated with indicated dilutions of antibodies e: Correlation graph showing the direct relationship between ACE2-GFP binding on S1-OFP beads with pseudovirion infection.
Article Snippet: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with
Techniques: Fluorescence, Incubation, Infection, Binding Assay